Deskripsi

Table of Contents

Part I Basic Methods in Stem Cell Culture 1

1 Human Embryonic Stem Cell Culture 3

Rudolfo Gonzalez, Robin L. Wesselschmidt,Philip H. Schwartz, and Jeanne F. Loring

2 Human Feeder Cells, Feeder-free, and Defined Culture Systems 18

Robin L. Wesselschmidt and Jeanne F. Loring

3 Mouse Embryonic Fibroblast Feeder Cells 34

Chris Stubban and Robin L. Wesselschmidt

4 Cryopreservation of Human Embryonic Stem Cells 47

Chris Stubban, Robin L. Wesselschmidt, Igor Katkov, and Jeanne F. Loring

Part II Characterization of Stem Cells 57

5 Classical Cytogenetics: Karyotyping 59

Robin L. Wesselschmidt and Jeanne F. Loring

6 Spectral Karyotyping and Fluorescent in situ Hybridization 71

Suzanne Peterson, Stevens Rehen, Willem Westra, Yun Yung, and Jerold Chun

7 Genotype and Epigenotype by Single Nucleotide Polymorphism (SNP) Analysis

85

Kevin Gunderson, David L. Barker, Marina Bibikova, and Jian-Bing Fan

8 Flow Cytometric Analysis of Human Embryonic Stem Cells 96

Andrew L. Laslett, Andrew Fryga, and Martin F. Pera

9 Immunocytochemical Analysis of Stem Cells 108

Lisa A. Flanagan, Boback Ziaeian, Theo Palmer, and Philip H. Schwartz

10 Characterization of Stem Cells Using Reverse Transcriptase Polymerase Chain Reaction

127

Shengwen Li, Ivan Kirov Jr., Henry J. Klassen, and Philip H. Schwartz

11 Gene Expression Profiling of Stem Cells by Microarray 149

Timothy McDaniel, Shawn Baker, Roy Williams, Franz-Josef Mueller, and David Barker

12 Generation of Human Embryonic Stem Cell-derived Teratomas 162

Robin L. Wesselschmidt

13 Characterization of Human Embryonic Stem Cell-derived Teratomas 171

Ivan Damjanov and Lars Ahrlund-Richter

Part III Differentiation of Human Embryonic Cells 183

14 Embryoid Bodies and Neuroepithelial Development 185

Matthew T. Pankratz and Su-Chun Zhang

15 Motor Neuron and Dopamine Neuron Differentiation 199

Xue-Jun Li, Dali Yang, and Su-Chun Zhang

16 Oligodendrocyte Differentiation from Human Embryonic Stem Cells 210

Maya N. Hatch, Gabriel I. Nistor, and Hans Keirstead

17 Cardiac Development of Human Embryonic Stem Cells 227

Maria Barcova, Victor M. Campa, and Mark Mercola

18 Hematopoiesis from Human Embryonic Stem Cells 238

Kausalia Vijayaragavan, Veronica Ramos-Meijia, and Mick Bhatia

Part IV Genetic Manipulation of Stem Cells 253

19 Genetic Manipulation of Human Embryonic Stem Cells: Lentivirus Vectors

255

Ruchi Bajpai and Alexey Terskikh

20 Genetic Manipulation of Embryonic Stem Cells 267

Tobias Raabe and Robin L. Wesselschmidt

Part V Advanced Methods 289

21 Derivation of Embryonic Stem Cells from Human Blastocysts 291

Prithi Rajan, David Smotrich, Ric Ross, Louise Laurent, and Jeanne F. Loring

22 Neural Progenitor Cell Culture 309

Hubert Nethercott, Heather Maxwell, and Philip H. Schwartz

23 Stem Cell Transplantation in the Brain 332

Jean-Pyo Lee, Nils Ole Schmidt, Paul Christian Baier, Franz-Josef Mueller, and Evan Y. Snyder

24 In Vitro Fertilization 351

Antoine La and David Diaz

25 Functional Characterization of Neurons 373

Scott McKerchner, Maria Talantova, and Stuart Lipton

Part VI Setting up a Laboratory 387

26 Setting up a Facility for Human Embryonic Stem Cell Research 389

Ian Lyons, David Tan, Philip H. Schwartz, and Mahendra Rao

Part VII Stem Cells and Society 415

27 Intellectual Property: Owning the Stem Cell 417

Cathryn Campbell and Jeanne F. Loring

28 Ethical Concerns for Stem Cell Research 426

Philip H. Schwartz and Michael W. Kalichman

29 Guidelines for Embryonic Stem Cell Research Oversight (ESCRO) Committees

437

Michael W. Kalichman and Philip H. Schwartz